Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV029737 | GenBank Accession | PP783754 |
| Subfamily | Parvovirinae | Genus | Erythroparvovirus |
| Species | Erythroparvovirus primate1 | Sequence Length | 1111 |
| Definition | Human parvovirus B19 isolate 320189 nonstructural protein (NS1) gene, partial cds. | Submission Date | 2024-05-07 |
| Strain Name | 320189 | Sampling Date | from 2010-01 to 2023-12 |
| Sampling Country | Israel | Location | Israel |
| Submitted By | Mor,O., Wax,M., Yitzhaki,M., Kriger,O., Zuckerman,N.S., Erster,O. and Arami,S.S. | Submitting Institution | Central Virology Laboratory, Ministry of Health, Chaim Sheba Medical Center, Ramat Gan 5262112, Israel |
| Original Host | Homo sapiens | Sample Type | whole blood or intrauterine samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Whole blood | Clinical samples used for B19V testing | 38543845 | View Related Publication |
| 2 | Intrauterine samples | Particularly in cases where diagnosis was requested for pregnant women | ||
| 3 | Custom-made RT-PCR | Molecular diagnosis of B19V | ||
| 4 | 1.1 kb PCR fragment | Generated for sequencing purposes, spans the NS1/VP1u region of the virus | ||
| 5 | Primers targeting nucleotides 1863–2953 of the NS1/VP1u B19V region | Used to amplify the 1.1 kb PCR fragment for sequencing | ||
| 6 | Nucleic acid extracts | Used for the initial RT-PCR diagnosis and subsequent sequencing | ||
| 7 | Illumina DNA prep library protocol | Library construction for next-generation sequencing (NGS) | ||
| 8 | MiSeq instrument | Used for NGS sequencing (Illumina, San Diego, CA, USA) |