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1
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RNAlater solution
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Used for storing fresh skin biopsies overnight at +4°C before long-term storage at ?20°C until analysis.
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38549497
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View Related Publication
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2
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QIAamp DNA or RNA kits (Qiagen)
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Used for the extraction and storage of total DNA and RNA from samples until analyses.
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3
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SYBR-Green qPCR
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Used for performing quantitative PCR on extracted RNA after RT-PCR step.
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4
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Benzonase endonuclease (Sigma-Aldrich)
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Used to determine whether the stool-derived viral DNA was encapsidated in viral particles or naked.
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5
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ASL buffer from the QIAamp DNA Stool Mini Kit (Qiagen)
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Used to suspend stool samples as part of the DNA extraction process.
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6
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Proteinase K
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Used as part of the DNA extraction process from stool samples.
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7
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Plasmids
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Served as naked DNA controls in the Benzonase endonuclease pretreatment experiment.
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8
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RNAscope ISH technology (Advanced Cell Diagnostics [ACD])
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Used for the detection of viral nucleic acid on FFPE tissue sections.
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9
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RNAscope 2.5 HD reagent kit-RED (ACD)
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Used to present hybridized targets as red dots in RISH.
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10
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RNase A (Qiagen)
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Used to differentiate between viral DNA and mRNA in tissue sections before RISH.
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11
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Anti-human CD3 clone OKT3 antibody (eBioscience)
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Used for immunohistochemical detection of T-cells.
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12
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Anti-human CD20 antibody, clone L26 (Biocare Medical)
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Used for immunohistochemical detection of B-cells.
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13
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Anti-Human CD68 antibody, clone KP1 (Fisher Scientific)
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Used for immunohistochemical detection of macrophages.
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14
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Anti-human Cytokeratin 10, clone RKSE60 (Abcam)
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Used for immunohistochemical detection of keratinocytes.
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15
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R-anti-CD4 antibody (Abcam; ab133616)
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Used for dual fluorescent IHC to stain CD4+ cells.
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16
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M-anti-CD8 antibody (Dako M7103)
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Used for dual fluorescent IHC to stain CD8+ cells.
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17
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4’,6-diamidino-2-phenylindole (DAPI)
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Used as a nuclear stain in dual fluorescent IHC.
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