ParvoDB ID PV029546 GenBank Accession PP460983
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate2 Sequence Length 4853
Definition Human bocavirus 2 isolate HBoV2/human/Taiwan/2019-11-28-4, partial genome. Submission Date 2024-03-07
Strain Name HBoV2/human/Taiwan/2019-11-28-4 Sampling Date from 2018 to 2022
Sampling Country China Location Taiwan, China
Submitted By Lin,J.-H., Chiu,S.-C., Yu,Y.-C. and Hu,S.-C. Submitting Institution Center of Diagnostics and Vaccine Development, Centers for Disease Control, No. 128, Sec. 2, Academia Rd., Nangang Dist., Taipei City 115201, Taiwan
Original Host Homo sapiens Sample Type Stool samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 taco Nucleic Acid Automatic Extraction System (GeneReach, Taichung City, Taiwan) For the extraction of viral DNA from multiple outbreaks. https://www.researchgate.net/publication/379528477_Human_Bocavirus_Circulating_in_Patients_With_Acute_Gastroenteritis_in_Taiwan_2018-2022 View Related Publication
2 AK-VP-F1 (5′-CGC CGT GGC TCC TGC TCT-3′) First-round PCR primer for detecting HBoV genotypes.
3 AK-VP-R1 (5′-TGT TCG CCA TCA CAA AAG ATG TG-3′) First-round PCR primer for detecting HBoV genotypes.
4 AK-VP-F2 (5′-GGC TCC TGC TCT AGG AAA TAA AGA G-3′) Second-round PCR primer for detecting HBoV genotypes.
5 AK-VP-R2 (5′-CCT GCT GTT AGG TCG TTG TTG TAT GT-3′) Second-round PCR primer for detecting HBoV genotypes.
6 1.5% agarose gel For electrophoresis to verify the PCR products.