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1
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A549 cells
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Used for virus isolation - inoculation in shell vials
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38257837
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View Related Publication
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2
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Highly pure intracellular viral DNA
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Used for molecular analysis - extracted from virus isolates
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3
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Heparin
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Used to collect plasma samples
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4
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EDTA
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Used to collect whole blood samples
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5
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Bam HI
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Used for preliminary species identification via DNA digestion
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6
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PCR amplification reagents
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Used for initial molecular typing - amplification of hexon hypervariable regions (HVR) 1–7 and fiber gene
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7
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Sanger sequencing reagents
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Used for sequencing hexon hypervariable regions (HVR) 1–7 and fiber gene
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8
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Illumina Miseq Instrument
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Used for next generation sequencing of purified viral DNA
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9
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Geneious Prime
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Software is excluded as per instructions, but listed as part of the annotation process
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10
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VAPiD
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For annotation of the resulting sequences
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11
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Bankit tool
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For uploading sequences to GenBank
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12
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MAFFT with FFT-NS-I setting
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For multiple sequence alignment
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13
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Simplot V3.5.1
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For sliding window analysis
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14
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Sac I
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Used for in silico restriction enzyme analysis to reveal a distinct profile for the identified virus
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15
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HHV6
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For monitoring HHV6 viremia during treatment
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16
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BK virus
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For monitoring BK virus-associated cystitis
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17
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Meropenem
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Used for treatment of ESBL-producing Klebsiella aerogenes infection
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18
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MACS GMP PepTivator AdV Select oligopeptides
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Used for the production of adenovirus-specific T cells (VSTs) - derived from various proteins of HAdV-C2 and -C5
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