Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV028965 | GenBank Accession | MZ546191 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 5400 |
| Definition | Bocaparvovirus sp. strain Parvozj-6, complete genome. | Submission Date | 2021-07-08 |
| Strain Name | Parvozj-6 | Sampling Date | 2016 |
| Sampling Country | China | Location | China |
| Submitted By | Yang,S., Zhang,W., Li,H. and Lu,H. | Submitting Institution | School of Medicine, Jiangsu University, 301, Xuefu Road, Zhenjiang, Jiangsu 212013, China |
| Original Host | Homo sapiens | Sample Type | fecal samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Disposable materials | Collection of fresh stool samples. | 35234620 | View Related Publication |
| 2 | Dry ice | Shipping of collected stool samples. | ||
| 3 | Phosphate-buffered saline (PBS) | Re-suspension of fecal samples. | ||
| 4 | Centrifuge tube (1.5 mL) | Collection of fecal supernatant after centrifugation. | ||
| 5 | Merck Millipore 0.45-μm filter | Filtration to remove bacterial and eukaryotic cell-sized particles. | ||
| 6 | Nuclease enzymes | Treatment of supernatant to digest unprotected nucleic acids. | ||
| 7 | QIAamp MinElute Virus Spin Kit (Qiagen) | Extraction of viral RNA and DNA. | ||
| 8 | Six base random primers | Synthesis of cDNA from viral RNA. | ||
| 9 | Klenow Fragment DNA polymerase (M0210L, New England Biolabs) | Generation of complementary DNA chain. | ||
| 10 | Nextera XT DNA Sample Preparation Kit (Illumina) | Construction of sequencing libraries. |