Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV028729 GenBank Accession AB691382
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 684
Definition Human parvovirus B19 NS1, VP1u genes for non-structural protein 1, VP1u capsid protein, partial cds, isolate: HP052. Submission Date 2012-01-06
Strain Name HP052 Sampling Date 1999-08
Sampling Country Japan Location Hokkaido, Japan
Submitted By Sakata,H. and Matsubayashi,K. Submitting Institution Contact:Hidekatsu Sakata Japanese Red Cross Hokkaido Block Blood Center; 2-1 Nijuyonken, Nishi-ku, Sapporo, Hokkaido 063-0802, Japan
Original Host Homo sapiens Sample Type blood sample
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 CL4800 testing system (Fujirebio, Inc., Tokyo, Japan) For CLEIA-B19V screening. 23145866 View Related Publication
2 Mouse monoclonal antibodies (MoAbs) Used in CLEIA-B19V in a two-step sandwich assay for detecting B19V antigen.
3 Magnetic particles Bound with one group of antibodies for CLEIA-B19V.
4 Alkaline phosphatase (ALP) Labeling another group of antibodies in CLEIA-B19V.
5 3(-2'-Spiroadamantane)-4-methoxy-4(-3''-phosphoryloxy)-phenyl-1,2-dioxetane Chemiluminescent substrate hydrolyzed by ALP to measure B19V antigen.
6 Virus spin kit (QIAamp MinElute, Qiagen K.K., Tokyo, Japan) Extraction of total nucleic acids from plasma samples.
7 2x universal probe master (FastStart, Rox; Roche Diagnostics Co., Ltd, Tokyo, Japan) For performing modified U-PCR for B19V.
8 Real-time PCR system (Model 7500, Applied Biosystems Japan Ltd, Tokyo, Japan) For real-time amplification in U-PCR for B19V.
9 TaqMan MGB probe Probe for detecting amplified B19V DNA in U-PCR.
10 Genotype 3a panels (M1655 and C2005) Provided for preliminary evaluation of U-PCR for B19V sensitivity.
11 World Health Organization (WHO) International Genotype Panel for B19V (NIBSC 09/110) Comprising three different members for evaluating sensitivity of U-PCR for various genotypes of B19V.
12 Six standard samples of B19V-positive plasma (Genotype 1) For quantitative assays calibrated using a commercial quantification kit.
13 Parvo-IgM and Parvo-IgG (Denka Seiken, Tokyo, Japan) For detecting anti-B19V antibodies (IgM and IgG) by enzyme-linked immunosorbent assays.
14 BigDye Terminator, Version 3.1 (Applied Biosystems) For cycle sequencing reactions.
15 Genetic analyzer (ABI 3100 or 3700, Applied Biosystems) For sequencing the DNA.
16 TaKaRa ExTaq HS (Takara Bio, Inc., Tokyo, Japan) DNA polymerase for performing PCR.
17 MagNA Pure LC system (Roche) Automated nucleic acid isolation system for isolating B19V DNA.
18 LightCycler Parvovirus B19V quantification kit (Roche) For measuring B19V DNA loads in pooled plasma.