Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV028058 GenBank Accession GU138991
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate2 Sequence Length 550
Definition Human bocavirus 2 isolate 52807 NP1 gene, partial cds. Submission Date 2009-10-26
Strain Name 52807 Sampling Date 2007-01
Sampling Country China Location Taiyuan Children Hospital, Taiyuan, Shanxi, China
Submitted By Xu,Z.Q. and Cheng,W.X. Submitting Institution Viral Diarrhea, Virology, 100 Yingxin Street, Beijing 100052, China
Original Host Homo sapiens Sample Type fecal samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 QIAamp viral RNA mini kit Extraction of viral DNA 21325551 View Related Publication
2 Recombinant DNA plasmid containing a partial region of the HBoV2 genome Template for PCR assay
3 Primers and probe Targeting the HBoV2 NP-1 gene for amplification
4 6-carboxyfluorescein Label for the 5' end of the TaqMan probe
5 Eclipse dark quencher Label for the 3' end of the TaqMan probe
6 pGEM-T Easy vector Cloning of the 587-bp DNA subgenomic fragment of HBoV2
7 QIAprep miniprep kit Purification of the plasmid
8 Eppendorf 6131 nuclear detector Quantification of the plasmid
9 Herring sperm DNA Used in Tris-EDTA buffer for preparing pGEM-T NP-1 HBoV2 plasmid dilutions
10 TaqMan Gene Expression Master Mix PCR reaction mix for DNA amplification
11 AmpliTaq Gold, UP enzyme PCR enzyme activated at 95°C
12 NS1 plasmid subclone Measuring the sensitivity of the nested PCR
13 Primers HBoV2-sf1, HBoV2-sr1, HBoV2-sf2, HBoV2-sr2 First and second round primers for nested PCR