Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV027764 | GenBank Accession | KC617868 |
| Subfamily | unclassified Parvoviridae | Genus | Parvo-like hybrid virus |
| Species | NIH-CQV | Sequence Length | 3780 |
| Definition | Parvovirus NIH-CQV putative 15-kDa protein, putative replication associated protein (rep), and putative capsid protein (cap) genes, complete cds. | Submission Date | 2013-02-14 |
| Strain Name | NIH-CQV | Sampling Date | 2007 |
| Sampling Country | China | Location | China |
| Submitted By | Xu,B., Zhi,N., Hu,G., Wan,Z., Zheng,X., Liu,X., Wong,S., Kajigaya,S., Zhao,K., Mao,Q. and Young,N.S. | Submitting Institution | Hematology, National Heart, Lung and Blood Institute, National Institute of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA |
| Original Host | Homo sapiens | Sample Type | serum samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | silica-binding spin columns used for nucleic acid extraction |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Solexa deep sequencing platform | To screen 10 pools of sera from patients for potential pathogens. | 23716702 | View Related Publication |
| 2 | Carrier RNA [synthetic poly(A)] | For nucleic acid extraction from digested samples. | ||
| 3 | Non-poly(A) random hexamers | To synthesize cDNA from extracted viral nucleic acids. | ||
| 4 | Invitrogen Platinum Taq PCR Buffer | For PCR to amplify overlapping DNA fragments for sequence verification. | ||
| 5 | Qiagen QIAamp MinElute Virus Kit | For DNA extraction for quantitative PCR. | ||
| 6 | Affinity-purified recombinant capsid protein | For immunoblotting to detect specific antibodies against NIH-CQV. | ||
| 7 | Mouse monoclonal antibody against polyhistidine tag | Used as a positive control in immunoblotting. | ||
| 8 | PageRuler Prestained Protein Ladder (Fermentas) | For molecular mass determination in immunoblotting. |