ParvoDB ID PV027762 GenBank Accession GQ455987
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 5297
Definition Human bocavirus strain FZ40, complete genome. Submission Date 2009-08-05
Strain Name FZ40 Sampling Date 2008-09-12
Sampling Country China Location China
Submitted By Xiu,W., Liu,G., Kang,Y., Shen,X., Xie,J., Wang,M., Zhang,W. and Zheng,K. Submitting Institution Viral Diseases, Fujian Center for Disease Control and Prevention, Jintai Road 76, Gulou Qu, Fuzhou, Fujian 350001, China
Original Host Homo sapiens Sample Type nasopharyngeal
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Viral Nucleic Acid Extraction kit VR100 For extracting viral nucleic acid from nasopharyngeal secretions http://jsu-mse.com/index.php/journal/article/viewFile/126/126 View Related Publication
2 Taq DNA polymerase (2.5 U) Used in PCR for DNA amplification
3 DNA Marker I Used as a molecular weight marker in gel electrophoresis
4 Gold ViewTM Nucleic Acid stain Used for staining nucleic acids in gel electrophoresis
5 dNTPs (10mmol/L) Used in PCR for DNA synthesis
6 Primers Primers used for PCR amplification
7 PowerPac basic electrophoresis apparatus Used for gel electrophoresis
8 MyCycler PCR amplification instrument Used for PCR amplification
9 Ultraviolet gel imaging system Used for visualizing nucleic acids in gel electrophoresis
10 Gel extraction and purification kit (wizard sV Gel and PCR Clean-Up system) Used for purifying PCR products from gel
11 Trypsin solution Used for liquefying mucus samples
12 RNase free water Used for collecting viral nucleic acid