Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV027411 | GenBank Accession | EU200667 |
| Subfamily | Parvovirinae | Genus | Tetraparvovirus |
| Species | Tetraparvovirus primate1 | Sequence Length | 5160 |
| Definition | Human parvovirus 4 strain HK1 non-structural protein (NS1), minor structural protein (VP1), and major structural protein (VP2) genes, complete cds. | Submission Date | 2007-10-10 |
| Strain Name | HK1 | Sampling Date | from 2005-08 to 2007-07 |
| Sampling Country | China | Location | Hong Kong, China |
| Submitted By | Woo,P.C.Y., Lau,S.K.P., Tse,H., Fu,C.T.Y., Au,W.-K., Tsoi,H.-W., Tsang,T.H.F., Chan,J.S.Y., Tsang,D.N.C., Tse,C.W.S., Ng,T.-K., Tsang,O.T.Y., Zheng,B.-J., Tam,S.C.F., Chen,X.-C., Zhou,B., Chan,K.-H. and Yuen,K.-Y. | Submitting Institution | Department of Microbiology, The University of Hong Kong, Pokfulam Road, Hong Kong, China |
| Original Host | Human | Sample Type | serum samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | QIAamp DNA Mini kit (Qiagen) | DNA extraction from all samples according to the manufacturer's protocol. | 18632954 | View Related Publication |
| 2 | forward primer 59-CCTGGTGCNGTNTGGAA-39 | PCR detection of parvoviruses targeting a 233 bp fragment within the VP2 region. | ||
| 3 | reverse primer 59-AAANATNGCATACTGGTTYA-39 | PCR detection of parvoviruses targeting a 233 bp fragment within the VP2 region. | ||
| 4 | QIAquick gel extraction kit (Qiagen) | Gel purification of PCR products. | ||
| 5 | ABI Prism 3700 DNA Analyser (Applied Biosystems) | Sequencing of both strands of the PCR products. | ||
| 6 | degenerate primers | Amplification of nearly full-length genome sequences of parvovirus-related viruses. | ||
| 7 | SuperScript III kit (Invitrogen) | Reverse transcription for the detection of PRRSV. | ||
| 8 | QIAamp Viral RNA Mini kit (Qiagen) | Extraction of viral RNA from swine samples. | ||
| 9 | forward primer LPW6922 59-CCTGTCGTCNGGNGAYCC-39 | PCR detection of PRRSV by amplifying a 147 bp fragment of the RNA-dependent RNA polymerase (RdRp) gene. | ||
| 10 | reverse primer LPW6923 59-ATGTCCTCRAAYTTNARYTG-39 | PCR detection of PRRSV by amplifying a 147 bp fragment of the RNA-dependent RNA polymerase (RdRp) gene. | ||
| 11 | Taq polymerase (Applied Biosystems) | PCR amplification in detection of PRRSV. |