1
Viral Transport Medium
Storage medium for collected nasal and throat swab samples.
20022295
2
3.2% Sodium Citrate
Anticoagulant in blood sample collection tubes.
3
QIAamp Viral RNA Mini Kit (QIAGEN, Hilden, Germany)
Extraction of total RNA and DNA from specimens.
4
RNase-free water
Elution of nucleic acids.
5
Takara Premix Ex TaqTM Perfect Real Time (Takara Biotechnology, Dalian, China)
Real-time PCR assays for the detection of HBoV.
6
Primers and Probe (targeted to the NP1 gene)
Used in real-time PCR assays to detect HBoV.
7
ABI PRISM 7900HT (Applied Biosystems, Foster City, CA, USA)
Equipment for running and analyzing real-time PCR assays.
8
pskHBoV containing the whole genome of HBoV ST2 genotype
Used as a standard for generating the standard curve in PCR.
9
One-Step RT-PCR kit (QIAGEN)
Conventional RT-PCR to detect HBoV and other respiratory viruses.
10
Multiplex RT-PCR reagent (described previously)
Diagnosis of other respiratory viruses in HBoV-positive samples.
11
QIAGEN’s One-Step RT-PCR kit (QIAGEN)
Amplification of the entire VP1 gene for sequencing.
12
QIAquick Gel Extraction Kit (QIAGEN)
Extraction of PCR product fragments for sequencing analyses.
13
pMD18T (Takara Biotechnology)
Cloning vector for sequencing.
14
Biosune Sequence Company and Life Biotechnology (Shanghai, China)
Services for sequencing the amplified products.
15
MEGA version 3.1 software
Software for phylogenetic analysis (excluded as it's software).
16
Spodoptera frugiperda Sf21 cells
Host cells for infection to recover recombinant baculovirus.
17
High Five cells
Cells infected with recombinant baculovirus to express VP2 proteins.
18
NaHCO3 (25 mM)
Solution for cell lysis.
19
Ammonium sulfate (20%)
Precipitation of proteins.
20
PBS (phosphate-buffered saline)
Resuspending proteins and dialysis.
21
pET30a (expression vector containing 6xHis tag)
Cloning and expression of VP2 fragments in E. coli.
22
E. coli BL21(DE3) host strains
Host strains for expression of recombinant proteins.
23
IPTG
Induction of protein expression in E. coli.
24
Ni-NTA agarose (Invitrogen)
Purification of His-tagged recombinant proteins.
25
SDS-PAGE gel (12.5%)
Separation of proteins for immunoblotting.
26
Nitrocellulose membranes
Transfer of proteins for Western blotting.
27
TBS-T (0.5% Tween-20 in TBS buffer)
Blocking and washing buffer during immunoblotting.
28
Nonfat dry milk (5%)
Blocking solution in immunoblotting.
29
Patient serum samples
Primary antibodies for detection in immunoblotting.
30
Anti-human IgG or IgM conjugated with horseradish peroxidase (HRP) (Bethy Laboratories, Montgomery, TX, USA)
Secondary antibodies for detection in immunoblotting.
31
3,3′-diaminobenzidine and H2O2
Substrates to reveal peroxidase activity in Western blot.
32
SARS-HCoV spike protein produced in E. coli
Negative control antigen for ELISA.
33
o-Phenylenediamine Dihydrochloride (OPD) (Sigma, St. Louis, MO, USA)
Substrate for HRP in ELISA.
