ParvoDB ID PV026513 GenBank Accession FJ265736
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 4846
Definition Human parvovirus B19 isolate P1 nonstructural protein 1 (NS1), 7.5 kDa protein, minor capsid protein (VP1), major capsid protein (VP2), and hypothetical 11 kDa protein genes, complete cds. Submission Date 2008-09-30
Strain Name P1 Sampling Date 2007-02
Sampling Country United States of America Location United States of America
Submitted By Rinckel,L.A., Buno,B.R., Gierman,T.M. and Lee,D.C. Submitting Institution NAT Development, Talecris Biotherapeutics, 1200 New Hope Road, Raleigh, NC 27610, USA
Original Host Homo sapiens Sample Type plasma samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Oligonucleotide primers and probes Used to develop a quantitative real-time PCR assay with broad specificity for B19V detection. 19389024 View Related Publication
2 Fluorophores Used to label detection probes in the PCR assay for visualization.
3 Plasmid pYT104-C (contains B19V Genotype 1 strain (Au) genome) Used as a quantitation standard in the real-time PCR assay.
4 First WHO International Standard for B19V DNA nucleic acid test assays (99/800) Used to determine the correlation factor for converting copies/mL to IU/mL in the real-time PCR assay.
5 Qiagen artus RealArt Parvo B19 PCR assay Used for additional testing of B19V-reactive samples.
6 B19V Immunoglobulin M (IgM) enzyme immunoassay (EIA) kits Used to detect IgM antibody levels in test samples.
7 B19V Immunoglobulin G (IgG) enzyme immunoassay (EIA) kits Used to detect IgG antibody levels in test samples.
8 Primers containing B19V consensus DNA sequences Used to generate PCR amplicons for DNA sequencing.