Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV026445 | GenBank Accession | ON334159 |
| Subfamily | Parvovirinae | Genus | Erythroparvovirus |
| Species | Erythroparvovirus primate1 | Sequence Length | 863 |
| Definition | Human parvovirus B19 isolate IR5 NS1 and VP1u genes, partial cds. | Submission Date | 2022-04-25 |
| Strain Name | IR5 | Sampling Date | from 2018-09 to 2019-10 |
| Sampling Country | Iran | Location | Mashhad, Khorasan, Iran, Iran |
| Submitted By | Rezaei,F., Keshavarz,M., Janati-Namin,N., Mokhtari Azad,T. and Arjeini,Y. | Submitting Institution | Bushehr University of Medical Sciences, The Persian Gulf Tropical Medicine Research Center, Sangi Blvd, Bushehr 7558113915, Iran |
| Original Host | Homo sapiens | Sample Type | Serum samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | ELISA kit (Euroimmune AG, Lübeck, Germany) | Measurement of serum levels of anti-B19V IgM and IgG antibodies in collected serum samples. | 36721508 | View Related Publication |
| 2 | ELISA kit (SERION ELISA AVIDITY, Germany) | Conducting IgG avidity test on IgG positive samples. | ||
| 3 | PVB-1 Primer | First-round PCR amplification of the NS1-VP1u region of B19V DNA. | ||
| 4 | PVB-3 Primer | Second-round PCR amplification to obtain the whole sequence of the target region. | ||
| 5 | B19SR Primer | Used in both first and second-round PCR to amplify the NS1-VP1u region of B19V DNA. | ||
| 6 | ABI 3130 genetic analyzer (Applied Biosystems, Foster City, CA) | Sequencing of the amplified product. | ||
| 7 | BigDye terminator cycle sequencing kit (Applied Biosystems) | Sequencing reagent in accordance with the manufacturer's instructions. |