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1
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Specific enzyme immunoassays (Biotrin International, France)
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To detect anti-Parvovirus IgG and IgM in sera
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17961236
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2
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LP 200 microplate reader (Diagnostic Pasteur, France)
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To read the colorimetric reaction in enzyme immunoassays
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3
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Proteinase K
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For digestion of sera to extract DNA
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4
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QIAamp DNA Blood kit (Qiagen, Hilden, Germany)
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To extract DNA from serum samples
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5
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Outer primers (CAAAAGCATGTGGAGTGAGG, CTACTAACATGCATAGGCGC)
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For the first round of nested PCR for B19 DNA detection
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6
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Inner primers (CCCAGAGCACCATTATAAGG, GTGCTGTCAGTAACCTGTAC)
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For the second round of nested PCR for B19 DNA detection
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7
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Taq DNA polymerase (Promega)
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For DNA amplification in PCR reactions
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8
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Molecular weight marker (100 bp ladder, Invitrogen)
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For estimating the size of PCR amplicons during agarose gel electrophoresis
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9
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QIAquick spin columns (Qiagen, Hilden, Germany)
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To purify nested PCR products from agarose gel bands
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10
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Taq Dideoxy Terminator cycle sequencing kit
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For sequencing the purified DNA
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11
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ABI Prism 377 DNA sequencer (Applied Biosystems)
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To perform DNA sequencing
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