Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV026181 GenBank Accession MZ681470
Subfamily Parvovirinae Genus Tetraparvovirus
Species Tetraparvovirus primate1 Sequence Length 87
Definition Human parvovirus 4 isolate IBTO-PARV4-211 minor capsid protein (VP1) gene, partial cds. Submission Date 2021-08-01
Strain Name IBTO-PARV4-211 Sampling Date from 2016-05 to 2016-06
Sampling Country Iran Location Tehran, Iran
Submitted By Ramezany,H., Kheirandish,M., Samiee,S. and Sharifi,Z. Submitting Institution Hematology and Blood Banking, High Institute for Research and Education in Transfusion Medicine, Iranian Blood Transfusion Organization (IBTO), Intersection of Sheikh Fazlollah Nouri Highway and Shahid Hemmat Highway, Tehran, Tehran 146651157, Iran
Original Host Homo sapiens Sample Type plasma specimens
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 HIV ELISA Kit (EIAgen Detect HIV 4 Total Screening Kit, Adaltis, Milan, Italy) Detection of anti-HIV antibodies in blood donors. 29663448 View Related Publication
2 HBsAg ELISA Kit (Enzygnost HBsAg 6.0, Siemens Healthcare Diagnostics Products GmbH, Marburg, Germany) Detection of HBsAg in blood donors.
3 Anti-HCV ELISA Kit (Enzygnost Anti-HCV 4.0, Siemens Healthcare Diagnostics Products GmbH, Marburg, Germany) Detection of antibodies against HCV in blood donors.
4 EUROIMMUN ELISA Kit (Lubek, Germany) Detection of specific B19 virus IgG and IgM antibodies in plasma specimens.
5 RTP DNA/RNA Virus Mini Kit (Invitek GmbH, Berlin, Germany) Extraction of B19-DNA from plasma.
6 Taq polymerase (YTA PCR Master Mix, Iran) DNA amplification in PCR.
7 PCR primers (PCO3 and PCO4) Amplification of β-globin gene to evaluate the suitability of extracted DNA.
8 PCR primers (F1, R1, F2, R2) Amplification of DNA sequence coding for the major capsid protein (VP2) of parvovirus B19.
9 Water and B19 plasmids Used as negative and positive controls, respectively, in PCR.
10 Ethidium bromide Staining of PCR products during agarose gel electrophoresis.
11 QIAquick PCR purification kit (Qiagen GmbH, Hilden, Germany) Purification of the nested PCR amplicons.
12 Artus parvo B19 RG PCR kit (Qiagen GmbH, Hilden, Germany) Quantitative Real-time PCR for the amplification and detection of B19 DNA.