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1
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HIV ELISA Kit (EIAgen Detect HIV 4 Total Screening Kit, Adaltis, Milan, Italy)
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Detection of anti-HIV antibodies in blood donors.
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29663448
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2
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HBsAg ELISA Kit (Enzygnost HBsAg 6.0, Siemens Healthcare Diagnostics Products GmbH, Marburg, Germany)
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Detection of HBsAg in blood donors.
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3
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Anti-HCV ELISA Kit (Enzygnost Anti-HCV 4.0, Siemens Healthcare Diagnostics Products GmbH, Marburg, Germany)
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Detection of antibodies against HCV in blood donors.
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4
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EUROIMMUN ELISA Kit (Lubek, Germany)
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Detection of specific B19 virus IgG and IgM antibodies in plasma specimens.
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5
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RTP DNA/RNA Virus Mini Kit (Invitek GmbH, Berlin, Germany)
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Extraction of B19-DNA from plasma.
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6
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Taq polymerase (YTA PCR Master Mix, Iran)
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DNA amplification in PCR.
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7
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PCR primers (PCO3 and PCO4)
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Amplification of β-globin gene to evaluate the suitability of extracted DNA.
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8
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PCR primers (F1, R1, F2, R2)
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Amplification of DNA sequence coding for the major capsid protein (VP2) of parvovirus B19.
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9
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Water and B19 plasmids
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Used as negative and positive controls, respectively, in PCR.
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10
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Ethidium bromide
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Staining of PCR products during agarose gel electrophoresis.
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11
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QIAquick PCR purification kit (Qiagen GmbH, Hilden, Germany)
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Purification of the nested PCR amplicons.
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12
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Artus parvo B19 RG PCR kit (Qiagen GmbH, Hilden, Germany)
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Quantitative Real-time PCR for the amplification and detection of B19 DNA.
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