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1
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EUROIMMUN ELISA kits
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Detection of B19 specific IgG and IgM antibodies in plasma samples.
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25771750
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View Related Publication
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2
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RTP DNA/RNA Virus Mini Kit (Invitek, Berlin, Germany)
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Extraction of viral DNA from plasma samples.
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3
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PCO3 primer
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Ampli?cation of b-globin gene to confirm the suitability of extracted DNA.
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4
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PCO4 primer
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Ampli?cation of b-globin gene to confirm the suitability of extracted DNA.
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5
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Taq polymerase (YTA PCR Master Mix, Iran)
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Polymerase chain reaction (PCR) to amplify DNA sequences.
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6
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1st PCR Reaction Primers (1 and 6)
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Detection of B19-DNA in the first step of nested PCR.
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7
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2nd PCR Reaction Primers (2 and 5)
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Detection of B19-DNA in the second step of nested PCR.
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8
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B19 primers P5F and P5R
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Amplification of a 349 bp fragment of B19 genomic DNA in the first-round of PCR.
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9
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B19 primers n-P5F and n-P5R
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Amplification of a 335 bp fragment of B19 genomic DNA in the second-round of PCR.
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10
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QIAquick PCR purification kit (Qiagen, Hilden, Germany)
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Purification of nested PCR products for sequencing.
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11
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100-bp DNA ladder (Sinaclon, Iran)
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Sizing of PCR products during agarose gel electrophoresis.
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12
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Ethidium bromide
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Staining DNA in agarose gel electrophoresis for visualization under UV light.
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13
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B19 plasmid
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Used as a positive control in PCR reactions.
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14
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Distilled water
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Used as a negative control in PCR reactions.
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