Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV026158 GenBank Accession KU891260
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 156
Definition Human parvovirus B19 isolate 7 minor structural protein (VP1) gene, partial cds. Submission Date 2016-03-09
Strain Name 7 Sampling Date from 2014-09 to 2014-11
Sampling Country Iran Location Tehran, Iran
Submitted By Ramezani,A., Aghakhani,A., Kazemimanesh,M., Banifazl,M. and Bavand,A. Submitting Institution Clinical Research Dept., Virology Dept., Pasteur Institute of Iran, Pasteur Institute of Iran (IPI), No. 69, 12th Farwardin Ave, Tehran, Tehran 1316943551, Iran
Original Host Homo sapiens Sample Type plasma samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 EUROIMMUN ELISA Kits Detection of B19 IgG and IgM antibodies in plasma samples. 28142135 View Related Publication
2 Dia.Pro Diagnostic BioProbes ELISA Kits Detection of hepatitis B surface antigen (HBsAg), hepatitis B core antibody (anti-HBc), and hepatitis C antibody (anti-HCV).
3 MP Biomedicals ELISA Kit Detection of human immunodeficiency virus (HIV) infection.
4 Diaplus Western Blot Analysis Confirmatory testing for HIV infection.
5 RTP DNA/RNA Virus Mini Kit (Invitek, Berlin, Germany) Extraction of B19-DNA from plasma samples.
6 PCO3 and PCO4 primers Amplification of a 110-bp fragment of the β-globin gene to evaluate the suitability of extracted DNA.
7 YTA PCR Master Mix PCR reaction mixture containing Taq polymerase for DNA amplification.
8 TJI and R4154 primers First round of nested PCR to amplify a fragment of the B19 VP2 gene.
9 TJ2 and TJII primers Second round of nested PCR for further amplification of the B19 VP2 gene.
10 SinaClon 100-bp DNA Ladder Size marker for electrophoresis of PCR products.
11 Ethidium Bromide Staining of agarose gels for visualization of DNA bands.
12 QIAquick PCR Purification Kit (Qiagen, Hilden, Germany) Purification of nested PCR products before sequencing.