Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV026090 | GenBank Accession | KM393166 |
| Subfamily | Parvovirinae | Genus | Erythroparvovirus |
| Species | Erythroparvovirus primate1 | Sequence Length | 5596 |
| Definition | Human parvovirus B19 isolate KU5, complete genome. | Submission Date | 2014-08-24 |
| Strain Name | KU5 | Sampling Date | 2013 |
| Sampling Country | United States of America | Location | Viracor Eurofins Laboratories (Lee’s Summit, MO), United States of America |
| Submitted By | Qiu,Y., Kleiboeker,S. and Li,B. | Submitting Institution | School of Medicine, University of Kansas Medical Center, 3901 Rainbow Blvd, Kansas City, KS 66160, USA |
| Original Host | Homo sapiens | Sample Type | plasma specimens |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Human plasma specimens | Source material containing the B19V genome. | 30533645 | View Related Publication |
| 2 | QIAamp DNA blood minikit (Qiagen) | Extraction of viral DNA from plasma specimens. | ||
| 3 | ABI 3730XL sequencer (MCLAB) | Sequencing of PCR-amplified DNA and the extracted DNA fragments. | ||
| 4 | AflII restriction enzyme | Cloning the left ITR. | ||
| 5 | PciI restriction enzyme | Cloning the right ITR. | ||
| 6 | BssHII restriction enzyme | Digesting positive clones in the middle of the ITR. | ||
| 7 | 1.5% agarose gel | Electrophoresis to separate the extracted DNA fragments. |