ParvoDB ID PV025910 GenBank Accession ON023023
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 5648
Definition Human parvovirus B19 isolate AA-26, complete genome. Submission Date 2022-03-18
Strain Name AA-26 Sampling Date 2015
Sampling Country Finland Location Finland
Submitted By Pyoria,L., Pratas,D., Toppinen,M., Hedman,K., Sajantila,A. and Perdomo,M.F. Submitting Institution Department of Virology, University of Helsinki, Haartmaninkatu 3, Helsinki 00290, Finland
Original Host Homo sapiens Sample Type Skin (right anterior femoral), brain (right frontal lobe), colon (ascending), liver (left lobe), lung (right upper lobe), heart (right atrium), kidney (right, superior segment), whole blood (right femoral vein) and pulled hair samples (scalp)
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 DNA Mini Kit (Qiagen) Total DNA extraction from tissue samples without RNAse treatment 36951096 View Related Publication
2 Proteinase K Enhances tissue lysis during DNA extraction
3 DL-dithiothreitol (DTT, Sigma Aldrich) Aids in the lysis of hair samples during DNA extraction
4 PBS Included as a negative control in DNA extraction
5 qPCR reagents Used for the quantification of viral DNAs (B19V, TTV, human herpesviruses, HBV, human polyomaviruses, and human single-copy gene RNase P)
6 HBV PCR Kit (Geneproof) Quantification of HBV DNA according to the manufacturer’s instructions
7 xGen Dual Index UMI Adapters (Integrated DNA Technologies) Used in DNA library preparation
8 KAPA HyperPlus library preparation kit (Roche) Preparation of DNA libraries for sequencing
9 myBaits Custom DNA-Seq (Arbor Biosciences) In-solution hybridization for vDNA capture during viral enrichment
10 MyBaits v5 kit (Arbor Biosciences) Supports the capture of vDNAs with low input DNA
11 xGen Universal Blockers-TS Mix (Integrated DNA Technologies) Blocks unspecific binding to adapters during hybridization
12 KAPA Library Quantification Kit (Roche) Quantification of DNA libraries before sequencing
13 KAPA Pure Beads (Roche) Used in clean-up steps during DNA library preparation and hybridization
14 Anti-HBc II, Anti-HBc IgM, and HbsAg Qualitative II kits (Abbott) Determination of the presence of hepatitis B core antibodies and surface antigen