ParvoDB ID PV025884 GenBank Accession KX788853
Subfamily Parvovirinae Genus Dependoparvovirus
Species Dependoparvovirus primate1 Sequence Length 558
Definition Adeno-associated virus isolate 6H nonfunctional capsid protein gene, partial sequence. Submission Date 2016-08-23
Strain Name 6H Sampling Date 2016-02-10
Sampling Country India Location India
Submitted By Purohit,N. and Rao,J.G. Submitting Institution Department of Biological Sciences and Bioengineering, Indian Institute Of Technology-Kanpur, Lab-13, Kanpur, Uttar Pradesh 208016, India
Original Host Homo sapiens Sample Type peripheral blood stem cells, bone marrow aspirates from acute promyelocytic leukemia cells, tissues samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Formalin Fixation of surgical excision tissues such as uterus, gall bladder, appendix, prostate, ovary, lymph node, liver, colon, ileum, endometrium, spleen, muscle, jejunum, urinary bladder, lung, tonsil, and kidney. 31419451 View Related Publication
2 Gentra Puregene Tissue kit Isolation of DNA from fresh or formalin-fixed and paraffin-embedded tissues.
3 FFPE Kit Isolation of DNA from formalin-fixed and paraffin-embedded tissues.
4 Hotstart PCR Mastermix Ready reaction mix for performing polymerase chain reaction (PCR).
5 NEB PCR mastermix Ready reaction mix for performing polymerase chain reaction (PCR).
6 Big Dye Terminator v3.1 Cycle Sequencing Kit Sequencing DNA samples displaying positive amplification for the AAV signature/entire capsid regions.
7 Stratagene QuickChange II XL Site-Directed Mutagenesis Kit Introduction of a single point mutation (S224A) in the AAV3 capsid encoding plasmid.
8 Iscove’s Modified Dulbecco’s Medium (IMDM) Culture medium used with and without Fetal Bovine Serum for vector transduction assays.
9 Fetal Bovine Serum (FBS) Supplement for complete IMDM used in cell culture for vector transduction assays.
10 Sodium bicarbonate Buffer supplement for IMDM in cell culture.
11 100X stock solution of antibiotics Antibiotic supplement (10,000 U penicillin + 10,000 mg streptomycin) for IMDM in cell culture.
12 Phosphate Buffered Saline (PBS) Diluent used in vector transduction assays and to mock-infect cells.