1
Formalin
Fixation of surgical excision tissues such as uterus, gall bladder, appendix, prostate, ovary, lymph node, liver, colon, ileum, endometrium, spleen, muscle, jejunum, urinary bladder, lung, tonsil, and kidney.
31419451
2
Gentra Puregene Tissue kit
Isolation of DNA from fresh or formalin-fixed and paraffin-embedded tissues.
3
FFPE Kit
Isolation of DNA from formalin-fixed and paraffin-embedded tissues.
4
Hotstart PCR Mastermix
Ready reaction mix for performing polymerase chain reaction (PCR).
5
NEB PCR mastermix
Ready reaction mix for performing polymerase chain reaction (PCR).
6
Big Dye Terminator v3.1 Cycle Sequencing Kit
Sequencing DNA samples displaying positive amplification for the AAV signature/entire capsid regions.
7
Stratagene QuickChange II XL Site-Directed Mutagenesis Kit
Introduction of a single point mutation (S224A) in the AAV3 capsid encoding plasmid.
8
Iscove’s Modified Dulbecco’s Medium (IMDM)
Culture medium used with and without Fetal Bovine Serum for vector transduction assays.
9
Fetal Bovine Serum (FBS)
Supplement for complete IMDM used in cell culture for vector transduction assays.
10
Sodium bicarbonate
Buffer supplement for IMDM in cell culture.
11
100X stock solution of antibiotics
Antibiotic supplement (10,000 U penicillin + 10,000 mg streptomycin) for IMDM in cell culture.
12
Phosphate Buffered Saline (PBS)
Diluent used in vector transduction assays and to mock-infect cells.
