Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV025734 | GenBank Accession | MH939296 |
| Subfamily | Parvovirinae | Genus | Tetraparvovirus |
| Species | Tetraparvovirus primate1 | Sequence Length | 734 |
| Definition | Human parvovirus 4 isolate P4_TBO748_SARI capsid protein (VP1) gene, partial cds. | Submission Date | 2018-09-21 |
| Strain Name | P4_TBO748_SARI | Sampling Date | 2018-03-19 |
| Sampling Country | India | Location | Virology Laboratory, Department of Microbiology, King George’s Medical University, Lucknow, Uttar Pradesh, India, India |
| Submitted By | Prakash,S., Shukla,S., Ramakrishna,V., Bhagat,A.K. and Jain,A. | Submitting Institution | Microbiology, King George's Medical University, Shahmina Road, Lucknow, Uttar Pradesh 226003, India |
| Original Host | Homo sapiens | Sample Type | The Nasal and Throat swab (NS/TS) samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Nasal and Throat swab (NS/TS) | Collect samples for testing respiratory viruses and PARV4 by real-time PCR. | 31605808 | View Related Publication |
| 2 | PureLink DNA/RNA mini kit (Invitrogen, USA) | Extract total viral nucleic acid from samples. | ||
| 3 | LightCycler 480 Probes Master (Roche Diagnostics, Nederland BV, Almere, the Netherlands) | Prepare master mix for TaqMan real-time PCR. | ||
| 4 | Parv4 forward and reverse primer (10 pm/ml) | Conduct real-time PCR specific to PARV4. | ||
| 5 | TaqMan probe (10 pm/ml) (IDT, USA) | Detect PARV4 in real-time PCR. | ||
| 6 | BigDye Terminator (BDT) cycle sequencing kit v3.1 (Applied Biosystems, Foster City, CA) | Conduct sequencing PCR for further analysis. | ||
| 7 | Exonuclease I and Shrimp Alkaline Phosphatase | Purify the nested PCR product before sequencing. | ||
| 8 | Ethanol | Purify sequencing PCR product. |