1
Enzyme immunoassay kits (EIARA1, Biomanguinhos; Premier Rotaclone1, Meridian Bioscience Inc; Ridascreen Rotavirus1, R-Biopharm)
Detection of group A rotavirus (RVA).
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2
Polyacrylamide gel electrophoresis (PAGE)
Detection of group A rotavirus (RVA).
3
QIAamp Viral RNA Mini Kit (QIAGEN1)
Extraction of viral DNA and RNA from stool samples.
4
High Capacity cDNA Reverse Transcription kit (Applied Biosystems)
Transcription of RNA to complementary DNA (cDNA).
5
QIAGEN QIAquick Gel Extraction Kit and PCR Purification Kit
Purification of PCR amplicons.
6
BigDye1 Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems)
DNA sequencing of PCR amplicons.
7
RVA primer sets (9con1, 9con2, 9T1-1, 9T1-2, 9T1-3P, 9T1-4, 9T-9B, FT5, 4con2, 4con3, 1T1, 1T1-Wa, 1T1-VN, 2T1, 3T1, 4T1, 5T1)
Genotyping of group A rotavirus (RVA).
8
NoV primer sets (Mon 432, Mon 434, Mon 431, Mon 433, COG 1, COG1R, RING1cP, COG 2F, COG2R, RING2P)
Detection and genotyping of noroviruses (NoV).
9
HAstV primer sets (Mon 269, Mon 270, AstVF, AstVR, AstVP)
Detection and genotyping of human astrovirus (HAstV).
10
HAdV primer sets (Hex1deg, Hex2deg, AdF, AdR, Adp1)
Detection and genotyping of human adenovirus (HAdV).
11
HBoV primer sets (AK-VP-F1, AK-VP-R1, AK-VP-F2, AK-VP-R2, HoV1F, HBoV1R, HBoV234F, HBoV 24R, HBoV3R, H1234probe)
Detection and genotyping of human bocavirus (HBoV).
12
TaqMan universal master mix1 (Applied Biosystems)
Quantitative PCR (qPCR) for detection and quantification of various enteric viruses.
