ParvoDB ID PV025620 GenBank Accession JQ513507
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 2016
Definition Human bocavirus isolate HBOV/Athens.GRC/589.08 capsid protein VP1 and capsid protein VP2 genes, complete cds. Submission Date 2012-01-26
Strain Name HBOV/Athens.GRC/589.08 Sampling Date 2008
Sampling Country Greece Location healthcare units of Southern Greece and in collaborating paediatric hospitals of Athens, Greece
Submitted By Pogka,V., Moutousi,A., Kossyvakis,A., Kalliaropoulos,A., Sgouras,D.N., Giannaki,M. and Mentis,A.F. Submitting Institution National Influenza Reference Laboratory of Southern Greece, Hellenic Pasteur Institute, Vas. Sofias AVE, Athens 11521, Greece
Original Host Homo sapiens Sample Type nasopharyngeal aspirates and/or throat swabs
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Viral transport medium (Mediaproducts BV, Groningen, The Netherlands) Transporting clinical specimens to the laboratory. 24373295 View Related Publication
2 Specific primers for HMPV (HMPV F1 and HMPV R1) Amplification of the genomic region corresponding to the full-length G protein coding region of HMPV.
3 Specific primers for HBoV (HBoV F1, HBoV R1, HBoV F2, HBoV R2, HBoV F3, HBoV R3, HBoV F4, HBoV R4) Amplification of the complete VP1/VP2 gene region of HBoV.
4 QIAquick PCR purification kit (Qiagen, Hilden, Germany) Purification of PCR products.
5 MinEluteTM gel extraction kit (Qiagen) Purification of PCR products.
6 GenomeLab DTCS-quick start sequencing kit (Beckman Coulter, Brea, CA, USA) Direct sequencing in both directions.