Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV025440 | GenBank Accession | MK211521 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 547 |
| Definition | Bocaparvovirus primate1 isolate NSVH2015-43-29214 capsid protein (VP1-3) gene, partial cds. | Submission Date | 2018-11-21 |
| Strain Name | NSVH2015-43-29214 | Sampling Date | 2015-10-19 |
| Sampling Country | Spain | Location | tertiary hospital in Barcelona (Catalonia, Spain), Spain |
| Submitted By | Pinana,M., Vila,J., Saura,J., Andres,C., Gimferrer,L., Esperalba,J., Codina,M.G., Martin,M.C., Fuentes,F., Rubio,S., Alcubilla,P., Rodrigo,C., Pumarola,T. and Anton,A. | Submitting Institution | Microbiology, Hospital Universitari Vall d'Hebron, Passeig de la Vall d'Hebron, 119-129, Barcelona 08035, Espanya |
| Original Host | Homo sapiens | Sample Type | respiratory specimens |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | NucliSENS easyMAG (BioMérieux, France) | Total nucleic acids extraction. | 36401674 | View Related Publication |
| 2 | GeneXpert Flu or Flu/RSV XC (Cepheid, USA) | Rapid real-time multiplex reverse transcription PCR (RT-PCR) for detecting HRSV and influenza A and B viruses during epidemics. | ||
| 3 | Sofia RSV FIA (Quidel, USA) | Immunofluorescent assay for detecting HRSV and influenza A and B viruses during epidemics. | ||
| 4 | D3 Ultra 8 DFA Respiratory Virus Screening & Identification kit (Diagnostic HYBRIDS, USA) | Immunofluorescence detection targeting FLUAV, FLUBV, HRSV, HAdV, HPIV 1, 2 and 3, and HMPV. | ||
| 5 | Anyplex II RV16 Detection or Allplex Respiratory Panel assays (Seegene, South Korea) | Real-time multiplex RT-PCR detection of FLUAV (with differentiation of H1, H1pdm09 and H3 subtypes), FLUBV, HRSV A and B, HAdV, EV, HMPV, HPIV-1, HPIV-2, HPIV-3, HPIV-4, HBoV, HCoV 229E, NL63 and OC43, and RV. | ||
| 6 | GoTaqG2 Green Master Mix (Promega Corporation, USA) | Nested PCR for partial sequencing of the HBoV VP1 gene. | ||
| 7 | Exo-SAP-IT (USB, Affymetrix Inc., Cleveland, USA) | Purification of inner PCR products. | ||
| 8 | ABI Prism BigDye Terminator v3.1 on the ABI PRISM 3130XL sequencer (Thermo Fisher Scientific, USA) | Sequencing of purified PCR products. |