Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV024175 GenBank Accession JN689408
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 651
Definition Human parvovirus B19 isolate 494 non-structural protein 1 (NS1) and VP1 protein (VP1) genes, partial cds. Submission Date 2011-09-16
Strain Name 494 Sampling Date 2005
Sampling Country Netherlands Location Netherlands
Submitted By van Binnendijk,R.S. and Boot,H.J. Submitting Institution Laboratory for Infectious Diseases and Perinatal Screening, National Institute for Public Health and the Environment (RIVM), P.O. Box 1, Bilthoven 3720 BA, The Netherlands
Original Host Homo sapiens Sample Type blood samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Roche LightCycler parvovirus B19 quantification assay Testing blood donations for presence of B19V DNA 22912828 View Related Publication
2 NucliSENS EasyMag extractor (BioMerieux) Extraction of B19V DNA from donor samples
3 NSoFw and Vp2oRev primers Primers for almost full genome PCR in B19V DNA amplification
4 2 units of long range PCR enzyme Enzyme component in PCR mixture for DNA amplification
5 500 mM of each deoxyribonucleotide triphosphate (dNTP) Component in PCR mixture for DNA amplification
6 1x Long range buffer containing 2.5 mM MgCl2 (Qiagen) Buffer component in PCR mixture for DNA amplification
7 ExoSAP-IT (USB) Purification of PCR products for sequencing reactions
8 Big Dye Terminator cycle sequencing (Applied Biosystems) Sequencing reactions of purified PCR products
9 B19V specific sequencing primers Primers for B19V sequencing reactions
10 ABI PRISM 3130xl Genetic Analyser (Applied Biosystems) Performing sequence analysis of B19V strains