1
ELISA kits (EIA-3504 and EIA-3503) from DRG Instruments GmbH, Germany
Used for the detection of anti-B19 IgM and anti-B19 IgG in serum samples.
29213152
2
Microtiter wells
Serve as a solid phase coated with recombinant Parvovirus B19 antigen (VP1 proteins) for ELISA.
3
Horseradish peroxidase (HRP)-conjugated anti-IgG and anti-IgM
Used to bind specific human anti-IgG or anti-IgM antibodies during the ELISA process.
4
Tetramethylbenzidine (TMB)
Used as a substrate for HRP, generating a blue color during the enzymatic reaction in the ELISA.
5
Sulfuric acid solution (H2SO4)
Stops the enzymatic reaction in ELISA, changing the color from blue to yellow.
6
Phenol
Part of the DNA extraction process for separating proteins from DNA.
7
Chloroform-isoamyl alcohol (24:1)
Used for separating proteins during DNA extraction from serum samples.
8
Ethanol (100% and 70%)
Employs in DNA precipitation and washing steps during DNA extraction.
9
Ammonium acetate (7.5 M)
Facilitates DNA precipitation during the extraction process.
10
Maxima Hot Start Taq DNA polymerase (Thermo Scientific)
Used for PCR amplification of the NS1 protein segment of Parvovirus B19 DNA.
11
GelRed (GelRed Nucleic Acid Gel Stain, Biotium)
Used for staining DNA in agarose gel electrophoresis.
12
Wizard SV Gel and PCR Clean-up system kit (Promega)
Utilized for purifying PCR products by centrifugation.
13
pCR 2.1-TOPO cloning vector (TOPO-TA Cloning kit, Invitrogen by Life Technologies)
Used for cloning the NS1 PCR product for transformation into E. coli.
14
One Shot TOP10 Competent Cells (Invitrogen by Life Technologies)
Chemically competent E. coli cells used for transformation with the cloned plasmid.
15
LB solid medium with kanamycin (50 μg/mL)
Used for growing transformed E. coli cells.
16
PureLink Quick Plasmid DNA Miniprep kit (Invitrogen by Life Technologies)
Purifies plasmid DNA from E. coli cultures.
17
TaqMan Fast Virus 1 step Master Mix (Applied Biosystems)
Used for real-time PCR amplification of viral DNA.
18
Maxima Probe/ROX master mix (Thermo Scientific)
Utilized for quantifying viral load in serum samples by real-time PCR.
19
Primers and probes described by Bonvicini et al.
Employed for real-time PCR targeting the NS1 gene of Parvovirus B19 genotype 1.
