Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV024144 GenBank Accession MH822929
Subfamily Parvovirinae Genus Protoparvovirus
Species Protoparvovirus primate3 Sequence Length 538
Definition Cutavirus isolate PA-pC VP2 gene, partial cds. Submission Date 2018-09-02
Strain Name PA-pC Sampling Date
Sampling Country Finland Location Finland
Submitted By Vaisanen,E., Fu,Y. and Soderlund-Venermo,M. Submitting Institution Department of Virology, Faculty of Medicine, University of Helsinki, Haartmaninkatu 3, Helsinki 00290, Finland
Original Host Homo sapiens Sample Type pre-carcinoma skin sample
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 QIAamp DNA FFPE Tissue Kit (Qiagen) DNA extraction from formalin-fixed and paraffin-embedded (FFPE) skin samples. 30239652 View Related Publication
2 QIAamp DNA Mini Kit (Qiagen) DNA extraction from freshly frozen skin biopsies.
3 AllPrep DNA/RNA/miRNA Universal Kit (Qiagen) DNA extraction from freshly frozen skin biopsies.
4 QIAamp DNA Blood Mini Kit (Qiagen) DNA extraction from serum samples.
5 AE buffer Elution of extracted DNA.
6 Maxima probe qPCR Master Mix (Thermo Scientific) without ROX PCR reactions for multiplex quantitative PCR (qPCR) to detect BuV, TuV, and CuV DNA.
7 Molecular biology-grade H2O To reach final volume in PCR reactions.
8 RNase P Quality control and cell-count quantification in qPCR.
9 Plasmids Used as positive controls and for generation of standard curves in qPCR.
10 Molecular biology-grade water Used as no-template controls in qPCR.
11 In-house BuV1-3-TuV-CuV-specific IgG EIAs IgG antibody assays to assess the seroprevalences of human protoparvovirus in serum samples.