Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV024139 GenBank Accession MH822924
Subfamily Parvovirinae Genus Protoparvovirus
Species Protoparvovirus primate3 Sequence Length 538
Definition Cutavirus isolate C53-CB VP2 gene, partial cds. Submission Date 2018-09-02
Strain Name C53-CB Sampling Date 2015
Sampling Country Finland Location Finland
Submitted By Vaisanen,E., Fu,Y. and Soderlund-Venermo,M. Submitting Institution Department of Virology, Faculty of Medicine, University of Helsinki, Haartmaninkatu 3, Helsinki 00290, Finland
Original Host Homo sapiens Sample Type CTCL skin sample
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 QIAamp DNA FFPE Tissue Kit (Qiagen) DNA extraction from formalin-fixed and paraffin-embedded (FFPE) skin samples. 30239652 View Related Publication
2 QIAamp DNA Mini Kit (Qiagen) DNA extraction from freshly frozen skin biopsies.
3 AllPrep DNA/RNA/miRNA Universal Kit (Qiagen) DNA extraction from freshly frozen skin biopsies.
4 QIAamp DNA Blood Mini Kit (Qiagen) DNA extraction from serum samples.
5 AE buffer Elution of extracted DNA.
6 Maxima probe qPCR Master Mix (Thermo Scientific) without ROX PCR reactions for multiplex quantitative PCR (qPCR) to detect BuV, TuV, and CuV DNA.
7 Molecular biology-grade H2O To reach final volume in PCR reactions.
8 RNase P Quality control and cell-count quantification in qPCR.
9 Plasmids Used as positive controls and for generation of standard curves in qPCR.
10 Molecular biology-grade water Used as no-template controls in qPCR.
11 In-house BuV1-3-TuV-CuV-specific IgG EIAs IgG antibody assays to assess the seroprevalences of human protoparvovirus in serum samples.