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1
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QIAamp DNA mini kit (Qiagen, Germany)
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Extraction of DNA from stool samples.
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27966636
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2
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Phusion High-Fidelity DNA Polymerase (Thermo Fischer Scientific)
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Amplification of the putative VP2 gene.
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3
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pFastBacDual vector (Invitrogen)
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Insertion of the VP2 amplicon for creating recombinant baculoviruses.
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4
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EZ-Link Sulfo-NHS-LC-Biotin kit (Thermo Fischer Scientific)
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Biotinylation of purified VP2 VLPs and insect cell lysate.
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5
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Complete-EDTA-free protease inhibitor cocktail (Roche)
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Protection of proteins during insect cell culture lysis.
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6
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Sodium deoxycholate (Sigma-Aldrich)
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Treatment of cell suspensions for protein extraction.
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7
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Tris-HCl buffer
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Cell lysis and protein purification for ultracentrifugation.
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8
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CsCl
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Creating a double cushion for ultracentrifugation and purifying VLPs.
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9
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PBS containing 0.05% Tween 20 (PBST)
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Buffer for immobilizing biotinylated VLPs in EIA assays.
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10
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Horseradish-peroxidase conjugated anti-human IgG (DAKO)
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Detection of human IgG in EIA assays.
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11
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3,3′,5,5′-tetramethylbenzidine (TMB, DAKO)
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Substrate for detecting horseradish peroxidase activity in EIA assays.
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12
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0.5 M H2SO4
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Stopping solution for TMB substrate reaction.
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13
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VP2 VLPs
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Antigens for BuV and TuV serology in ELISA assays.
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14
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High5 insect cells
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Expression of VP2 VLPs for use as control in ELISA assays.
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15
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Streptavidin-coated plates (UniverSA96-Lockwell, Kaivogen)
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Immobilization of biotinylated VLPs for ELISA detection.
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16
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Diluent (Labsystems Diagnostics)
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Minimizing non-specific background in ELISA assays.
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17
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Soluble VLPs and insect cell lysate
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Blocking agents in competition assays for confirming IgG results.
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