Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV024124 | GenBank Accession | KJ856908 |
| Subfamily | Parvovirinae | Genus | Erythroparvovirus |
| Species | Erythroparvovirus primate1 | Sequence Length | 257 |
| Definition | Human parvovirus B19 isolate SNHRC-B19-002 from India capsid protein (VP1/VP2) gene, partial cds. | Submission Date | 2014-05-19 |
| Strain Name | SNHRC-B19-002 | Sampling Date | 2013-11-04 |
| Sampling Country | India | Location | India |
| Submitted By | Vadivel,K., Ramamurthy,M., Sankar,S., Sagadevan,K., Nandagopal,B. and Sridharan,G. | Submitting Institution | Sri Sakthi Amma Institute of Biomedical Research, Sri Narayani Hospital and Research Centre, Sripuram, Vellore, Tamil Nadu 632055, India |
| Original Host | Homo sapiens; non-traumatic arthropathies | Sample Type | serum |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Qiagen DNA Blood Mini Kit (Qiagen, Hilden, GmBH, Germany) | Extraction of DNA from serum samples as per the manufacturer’s instruction. | 29998875 | View Related Publication |
| 2 | Serum | Sample type used for nested PCR (nPCR) assay. | ||
| 3 | Plasmid construct | Used as a quantitative positive control in the nested PCR assay. | ||
| 4 | DNAse-free water, Qiagen Buffer, deoxynucleotides (1 mM), forward and reverse primers (25 pmol) (Eurofins, Bangalore), HotStar Taq polymerase (2 U) | Components of the nPCR mix. | ||
| 5 | 2 per cent agarose (Sigma, MO, USA) | Used for agarose gel electrophoresis to detect the amplified product. | ||
| 6 | 1X Tris-acetate-EDTA buffer | Buffer used in agarose gel electrophoresis. | ||
| 7 | Ethidium bromide (Sigma, St. Louis, USA) | Staining agent used for visualization in agarose gel electrophoresis. | ||
| 8 | TOPO TA Cloning Kit (Invitrogen, CA) | Used to clone the nPCR product for validation of the assay. |