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1
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Medium 199 (Invitrogen)
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Component of hMEC-1 medium
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https://d-nb.info/1162844299/34
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View Related Publication
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2
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FCS (Ftales Klberserum, PAA)
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Supplement for hMEC-1 medium and UT-7/EPO medium
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3
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Penicillin/Streptomycin (Pen/Strep, PAA)
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Antibiotic supplement for hMEC-1 medium and UT-7/EPO medium
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4
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Hydrocortison (Invitrogen)
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Supplement for hMEC-1 medium
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5
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ECGS (Endothelial cell growth supplement, BD Biosciences)
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Supplement for hMEC-1 medium
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6
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RPMI (PAA)
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Component of UT-7/EPO medium
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7
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Trypsin/EDTA
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Detachment of cells from culture flasks
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8
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NucleoSpin RNA II (Macherey & Nagel)
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Isolation of total RNA
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9
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DNase (Ambion)
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Removal of genomic DNA from RNA samples
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10
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Geneticin (G418, Biochrom)
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Selection of stable cell lines
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11
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Doxycyclin (Sigma)
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Induction of gene expression
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12
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LB-Medium/LB-Agar (BD, Difco, Merck)
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Bacterial culture medium
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13
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Phenol/Chloroform/Isoamylalkohol (Fluka)
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DNA extraction
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14
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Proteinase K (Roth)
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Lysis of cells for DNA extraction
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15
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Agarose
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Gel electrophoresis
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16
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TaqMan Universal PCR Master Mix (Applied Biosystems)
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qPCR reagent
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17
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Tryptone, Hefeextrakt, NaCl, NaOH (BD, Merck)
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Components of LB medium
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18
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10× PBS (Merck, Roth, Sigma)
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Phosphate buffered saline solution
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19
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Dimethylsulfoxid (DMSO; Serva Electrophoresis)
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Component of cryomedium
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20
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Saccharose (Roth)
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Preparation of a sucrose cushion for virus particle purification
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21
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Oligonucleotides (MWG Biotech AG)
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Primers for PCR and qPCR
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