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1
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Flocked swabs
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To collect and transport respiratory samples in liquid viral transport medium.
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22028039
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2
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Liquid viral transport medium (Copan Italia S.p.A., Brescia, Italy)
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To transport respiratory samples.
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3
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Total nucleic acid isolation kit (Roche Applied Science, Mannheim, Germany)
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To carry out automatic nucleic acid extraction.
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4
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Equine herpesvirus 1 (EHV1)
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Added as an internal control during nucleic acid extraction.
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5
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Platinum Quantitative PCR SuperMix-UDG kit (Invitrogen, Carlsbad, CA)
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To perform multiplex real-time PCR for HBoV and EHV1.
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6
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StepOne instrument (Applied Biosystems, Foster City, CA)
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To conduct PCR amplification.
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7
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Monoclonal antibodies (Oxoid, Cambridge, UK)
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To detect antigens of adenovirus (AdV), RSV, influenza viruses A and B, and parainfluenza viruses 1-3 by direct immunofluorescence assay (DFA).
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8
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Promega PCR Master Mix (Promega, Madison, WI)
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To amplify the 1,136 bp VP1/VP2 gene region of the HBoV genome for sequencing.
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9
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BigDye terminator chemistry (Applied Biosystems)
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To purify and sequence PCR products using an ABI PRISM 310 genetic analyzer.
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