ParvoDB ID PV024081 GenBank Accession GU989638
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 1136
Definition Human bocavirus isolate Slovenia-9 VP1/VP2 gene, partial cds. Submission Date 2010-03-10
Strain Name Slovenia-9 Sampling Date 2010-02-24
Sampling Country Slovenia Location Slovenia
Submitted By Ursic,T. and Petrovec,M. Submitting Institution Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloska 4, Ljubljana SI-1000, Slovenia
Original Host Homo sapiens Sample Type asopharyngeal swab, tracheal aspirate and blood sample
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 short-acting beta-2 agonist To treat respiratory distress. 21227992 View Related Publication
2 parenteral steroid To treat respiratory infection.
3 amoxicillin-clavulanic acid To treat respiratory infection.
4 saline To support circulatory stability.
5 dopamine To support circulatory stability.
6 concentrated erythrocytes To treat low hemoglobin concentration.
7 MagNA pure compact instrument For automatic nucleic acid extraction.
8 total nucleic acid isolation kit For automatic nucleic acid extraction.
9 platinum quantitative PCR SuperMix-UDG kit For real-time qPCR.
10 Promega PCR MasterMix To amplify a region of the HBoV genome.
11 BigDye terminator chemistry For sequencing PCR products.
12 sequencing primers To achieve appropriate sequence coverage.
13 nasopharyngeal swab To collect samples for testing.
14 blood sample To assess the etiology of the respiratory disease.
15 tracheal aspirate To assess the etiology of the respiratory disease.
16 bronchoalveolar (BAL) fluid To set up cultures for detecting bacterial and fungal pathogens.
17 thoracal drainage fluid To set up cultures for detecting bacterial and fungal pathogens.
18 hemoculture To set up cultures for detecting bacterial and fungal pathogens.
19 phosphotungstic acid (PTA) To stain EM preparations.
20 protein A gold (15 nm) For immunoelectron microscopy.