Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV024072 GenBank Accession JQ995603
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 213
Definition Human bocavirus strain HIMFG525 nonstructural protein-like gene, partial sequence. Submission Date 2012-04-30
Strain Name HIMFG525 Sampling Date 2010-04-15
Sampling Country Mexico Location Mexico
Submitted By Uribe-Gutierrez,G., Hernandez-Santos,H., Moreno-Espinosa,S., Nava-Frias,M., Gomez-Ortega,M.R., Olvera-Rosete,D.P., Manjarrez-Zavala,M.E., Gonzalez-Marquez,H., Fierro-Pastrana,R.C. and Arellano-Galindo,J. Submitting Institution Laboratorio de Virologia, Hospital Infantil de Mexico Federico Gomez. Dr. Marquez # 162. Col.Doctores, Del. Cuauhtemoc., Doctorado en Ciencias Biologicas y de la Salud, Universidad Autonoma Metropolitana, Mexico, Calzada del Hueso 1100 Col. Villa Quietud, Delegacion Coyoacan C. P. 04960, Mexico, DF 06720, Mexico.
Original Host Homo sapiens Sample Type nasopharyngeal swab
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 137 mM NaCl, 5.4 mM KCl, 0.25 mM Na2HPO4, 0.44 mM KH2PO4, 1.3 mM CaCl2, 1 mM MgSO4, and 4.2 mM NaHCO3 Transport medium for swab samples 25185412 View Related Publication
2 Commercial kit (cat. no. 3105; Light Diagnostics, Temecula, CA, USA) Immunofluorescence staining for detection of AdV
3 Minimal essential medium (cat. no. 61100-061; Gibco Life Technologies, Grand Island, NY, USA) Growing A549 cells for virus isolation
4 Foetal bovine serum (cat. no. 104380-26; Gibco Life Technologies) Supplement for minimal essential medium
5 0.22 μm filter Filtering samples for culturing
6 Reference HAdV C VR-1078AS/RB ATCC VR1, type 1 Control in virus isolation experiments
7 10 mg/mL proteinase K DNA digestion for extraction
8 Phenol-chloroform Purifying DNA from clinical samples
9 QIAamp DNA Mini Kit 50 (cat. no. 51304; Qiagen, Germantown, MD, USA) DNA extraction for diagnosis of HBoV
10 Buffer 10X (cat. no. B9004S; New England Biolabs, Ipswich, MA, USA) Component of PCR mixture for AdV detection
11 200 μM dNTPs (cat. no., U1240; Promega, Madison, WI, USA) Component of PCR mixture for AdV detection
12 1.5 mM MgCl2 (cat. no. M1028-10X1ML; Sigma-Aldrich, St. Louis, MO, USA) Component of PCR mixture for AdV detection
13 Q-Solution 1x Component of PCR mixture for HBoV detection
14 1.5 units of Taq DNA polymerase Component of PCR mixture for HBoV detection