1
Stool collection tubes
Collection and transfer of stool samples for lab analysis.
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2
Phosphate-buffered saline with glycerol (15%)
Dilution buffer for stool samples.
3
AmpliSensRIBO-sorb extraction kit
Total viral nucleic acid extraction.
4
AmpliSensReverta-L reverse-transcription kit
Synthesis of complementary DNA.
5
DreamTaq Green PCR Master Mix (Thermo Scientific, Lithuania)
PCR assays.
6
Mon244/245 primers
Detection of HAstV by amplifying a 413-bp fragment of the ORF2.
7
Agarose gel with ethidium bromide
Electrophoresis separation and visualization of PCR products.
8
Gel Doc XR+Imager System (Bio-Rad Laboratories, Inc.)
Examination of electrophoresis results.
9
Beg9/End9 primers
Amplification and sequencing of the VP7 gene of RVA.
10
4Con3 and 4Con2 primers
Sequencing of the VP4 gene of RVA.
11
VP6F/VP6R and End6 primers
Sequencing of partial VP6 gene of RVA.
12
JV12/JV13, CVR
Sequencing of NoV including the ORF1/ORF2 overlapping region.
13
Mon381F/383R primers
Sequencing of NoV capsid genotype.
14
BigDyeTerminator v.3.1 Cycle Sequencing Kit
Determination of nucleotide sequences.
15
ABI 3500 Genetic Analyzer (Applied Biosystems, CA, USA)
Sequencing analysis.
16
SeaKem GTG-agarose gel (Lonza, ME, USA)
Purification of PCR products.
1
Ribo-Sorb (Interlabservis, Russia) extraction kit
Isolation of viral DNA from fecal samples
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2
Primers HBoV2-sf2 (50-TGCTTCAACAGGCAAAACAA-30) and HBoV2-sr2 (50-TCCAAGAGGAAATGAGTTTGG-30)
Detection of bocavirus DNA by polymerase chain reaction (PCR)
3
Silica Bead DNA Gel Extraction kit (Fermentas)
Purification of PCR-amplified fragments
4
BigDye Terminator v. 3.1 Cycling Sequencing kit (Applied Biosystems)
Sequencing of purified PCR products
5
CentriSep (Applied Biosystems) columns
Purification of sequencing reaction products
6
MEGA 5.0 software (Tamura et al., 2011)
Analysis of sequences (Note: This is a software)
7
BioEdit v. 7.0
Sequence alignment and pairwise nucleotide identities
8
ClustalX v. 1.8
Sequence alignment
9
RDP3 program
Identification of recombinant strains (This is software)
10
BEAST package
Molecular evolutionary rate estimation and inference of maximum clade credibility trees
11
Uranyl acetate
Negative staining for electron microscopy
