1
Stool collection tubes
Collection and transfer of stool samples for lab analysis.
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2
Phosphate-buffered saline with glycerol (15%)
Dilution buffer for stool samples.
3
AmpliSensRIBO-sorb extraction kit
Total viral nucleic acid extraction.
4
AmpliSensReverta-L reverse-transcription kit
Synthesis of complementary DNA.
5
DreamTaq Green PCR Master Mix (Thermo Scientific, Lithuania)
PCR assays.
6
Mon244/245 primers
Detection of HAstV by amplifying a 413-bp fragment of the ORF2.
7
Agarose gel with ethidium bromide
Electrophoresis separation and visualization of PCR products.
8
Gel Doc XR+Imager System (Bio-Rad Laboratories, Inc.)
Examination of electrophoresis results.
9
Beg9/End9 primers
Amplification and sequencing of the VP7 gene of RVA.
10
4Con3 and 4Con2 primers
Sequencing of the VP4 gene of RVA.
11
VP6F/VP6R and End6 primers
Sequencing of partial VP6 gene of RVA.
12
JV12/JV13, CVR
Sequencing of NoV including the ORF1/ORF2 overlapping region.
13
Mon381F/383R primers
Sequencing of NoV capsid genotype.
14
BigDyeTerminator v.3.1 Cycle Sequencing Kit
Determination of nucleotide sequences.
15
ABI 3500 Genetic Analyzer (Applied Biosystems, CA, USA)
Sequencing analysis.
16
SeaKem GTG-agarose gel (Lonza, ME, USA)
Purification of PCR products.
1
PBS–glycerin buffer
Clarifying stool specimen for viral DNA isolation.
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2
Ribo-Sorb (Interlabservis) extraction kit
Isolating viral DNA from stool specimens.
3
Primers sf2 and sr2
Detecting all HBoV genotypes during PCR.
4
DreamTaq Buffer (ThermoScientific)
Providing the necessary ions and pH for the PCR reaction.
5
dNTPs
Building blocks for DNA synthesis during PCR.
6
Primers (25 pmol of each)
Starting points for DNA synthesis during PCR.
7
DreamTaq DNA Polymerase (ThermoScientific)
Catalyzing the synthesis of DNA during PCR.
8
Agarose gel (1.5%)
Analyzing amplification products via electrophoresis.
9
RIBO-sorb Extraction Kit (Central Research Institute of Epidemiology, Russia)
Extracting DNA for detecting enteric viruses and bacteria.
10
Reverta-L reverse-transcription kit (Central Research Institute of Epidemiology, Russia)
Reverse-transcribing RNA to DNA for virus detection.
11
Rotavirus/Norovirus/Astrovirus PCR kits (Central Research Institute of Epidemiology, Russia)
Detecting RNA of group A rotavirus, norovirus genogroup II, and human astrovirus.
12
Enterovirus-FL PCR kit (Central Research Institute of Epidemiology, Russia)
Detecting enterovirus RNA.
13
Ampli- Sens Shigella spp. and EIEC/Salmonella spp./Campylobacter spp.-FL PCR kits (Central Research Institute of Epidemiology, Russia)
Detecting DNA of Salmonella spp., Campylobacter spp., Shigella spp., and enteroinvasive Escherichia coli (EIEC).
14
DNA Gel Extraction kit (Fermentas)
Purifying amplified fragments for sequencing.
15
BigDye Terminator v. 3.1 Cycling Sequencing kit (Applied Biosystems)
Sequencing the purified DNA fragments.
16
CentriSep columns (Applied Biosystems)
Purifying reaction products for sequencing.
