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1
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Peripheral blood mononuclear cells (PBMCs)
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To investigate the presence of B19V DNA and mRNA in different cell fractions.
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25805750
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2
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CD34+KDR+ cells
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Magnetically sorted cell fraction to quantify B19V DNA and to investigate viral replication.
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3
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CD34+ cells
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Isolate hematopoietic progenitor cells for comparison with CD34+KDR+ and CD34- cells.
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4
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CD34- cells
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Isolate lineage-committed cell fraction for comparison with CD34+KDR+ and CD34+ cells.
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5
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Early outgrowth EPC (eo-EPC)
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Cultivated from bone marrow and blood to quantify numbers and investigate B19V DNA presence.
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6
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Endothelial Colony-Forming Cells (ECFC)
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To analyze the infectibility and B19V gene expression after in vitro infection.
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7
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Colony Forming Unit-Hill (CFU-Hill) colonies
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Cultivated to study their infectibility and changes after B19V infection.
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8
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Erythroblastoid cell line UT7/Epo-S1
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Used as a comparison for in vitro infectibility with B19V.
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9
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Quantitative PCR
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To quantify B19V DNA from sorted cell fractions and endomyocardial biopsy samples.
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10
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In situ hybridization
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To prove the intracellular presence of B19V after infection in vitro.
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11
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RT-PCR
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To detect B19V gene transcripts (NS1, VP1/VP2, 11kDa) in infected CAC.
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12
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FACS analysis
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To analyze receptor and coreceptor expression on CAC and to quantify apoptotic markers like active caspase-3 and annexin V.
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13
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Annexin V
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To identify apoptotic cells post B19V infection.
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14
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Caspase-3 antibody
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To detect active caspase-3 in apoptotic CAC after B19V infection.
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15
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TUNEL assay
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To detect terminal apoptosis in B19V-infected CAC.
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16
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EVCB (EndoCult Liquid Medium Kit)
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To cultivate CFU-Hill colonies.
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17
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Evans Blue
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To quantify reendothelialization in a murine carotid artery injury model.
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18
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Class I Human Leukocyte Antigen (HLA-I)
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To detect human eo-EPC in murine carotid arteries post-transplantation.
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19
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Anti-mouse CD117-APC and CD309-PE antibodies
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To quantify endogenous murine progenitors by FACS analysis post carotid injury.
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