Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV023472 | GenBank Accession | MT873798 |
| Subfamily | Parvovirinae | Genus | Tetraparvovirus |
| Species | Tetraparvovirus primate1 | Sequence Length | 161 |
| Definition | Human parvovirus 4 isolate pool-14 NS1 gene, partial cds. | Submission Date | 2020-08-10 |
| Strain Name | pool-14 | Sampling Date | 2017 |
| Sampling Country | China | Location | Central China, China |
| Submitted By | Zhong,Y., Jia,J. and Ma,Y. | Submitting Institution | NMPA Key Laboratory for Quality Control of Blood Products, Institute of Health Service and Transfusion Medicine, Academy of Military Medical Sciences, No. 27 Taiping Road, Haidian District, Beijing, Beijing 100850, China |
| Original Host | Homo sapiens | Sample Type | plasma sample |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | High Pure Viral Nucleic Acid Kit (Roche Diagnostics) | Isolate Viral DNA from plasma samples. | 33200412 | View Related Publication |
| 2 | GeneQuant 1300 spectrophotometer (GE Healthcare Bio‐sciences AB) | Measure concentration and purity of extracted DNA. | ||
| 3 | qPCR assay | Detect and quantify B19V and PARV4 DNA. | ||
| 4 | Standard plasmids containing qPCR target sequences | Generate standard curve for quantifying virus DNA. | ||
| 5 | Nested PCR primers | Amplify 161-bp region related to the NS1 gene of PARV4. | ||
| 6 | pMD18‐T vector (TaKaRa Bio) | Clone PCR amplification products for sequencing. | ||
| 7 | ABI 3730XL DNA Analyzer | Sequence PCR products. |