Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV023404 GenBank Accession DQ353696
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 354
Definition Human bocavirus isolate BJ3111 nonstructural protein 1 gene, partial cds. Submission Date 2006-01-05
Strain Name BJ3111 Sampling Date from 2010-11 to 2011-10
Sampling Country China Location Affiliated Children's Hospital of the Capital Institute of Pediatrics, China
Submitted By Zhao,L., Qian,Y., Zhu,R., Deng,J., Wang,F. and Li,Y. Submitting Institution Department of Virology, Capital Institute of Pediatrics, 2 YaBao Road, Beijing 100020, China
Original Host None Sample Type Faecal samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Phosphate buffer Diluting stool samples 23133667 View Related Publication
2 DNAzolH BD (Molecular Research Center, USA) DNA extraction from supernatants of stool samples
3 8 mM NaOH Re-suspending extracted DNA
4 TaqMan Universal Master Mix (Applied Biosystems, USA) Real-time PCR reaction mix
5 Diethyl-pyrocarbonate (DEPC)-treated water Preparation of real-time PCR reaction mix
6 pGEM-T NS1 HBoV2 plasmid Positive control in real-time PCR
7 Phosphate buffer Control in nucleic acid extraction and PCR amplification
8 26 GreenMix (Promega, USA) PCR reaction mix
9 DNAase/RNAase free water Preparation of PCR reaction mix
10 EasyPure Quick Gel extraction kit (TransGen Biotech, Beijing, China) PCR product purification
11 pGEM-T vector Ligation of PCR products for sequencing
12 DNA ligation kit (Promega, USA) Ligation of PCR products for sequencing
13 Escherichia coli strain DH5α Transformation for sequencing
14 DEPC-treated water Negative control in real-time PCR