Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV023342 GenBank Accession JX257046
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate2 Sequence Length 5307
Definition Human bocavirus 2 strain BJQ435, complete genome. Submission Date 2012-06-28
Strain Name BJQ435 Sampling Date 2011-07-25
Sampling Country China Location Beijing, China
Submitted By Zhao,H., Zhao,L., Sun,Y., Qian,Y., Liu,L., Jia,L., Zhang,Y. and Dong,H. Submitting Institution Laboratory of Virology, Capital Institute of Pediatrics, 2 Yabao Road, Beijing 100020, China
Original Host Homo sapiens Sample Type fecal samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Phosphate buffer Diluting stool samples 23133667 View Related Publication
2 DNAzolH BD (Molecular Research Center, USA) DNA extraction from supernatants of stool samples
3 8 mM NaOH Re-suspending extracted DNA
4 TaqMan Universal Master Mix (Applied Biosystems, USA) Real-time PCR reaction mix
5 Diethyl-pyrocarbonate (DEPC)-treated water Preparation of real-time PCR reaction mix
6 pGEM-T NS1 HBoV2 plasmid Positive control in real-time PCR
7 Phosphate buffer Control in nucleic acid extraction and PCR amplification
8 26 GreenMix (Promega, USA) PCR reaction mix
9 DNAase/RNAase free water Preparation of PCR reaction mix
10 EasyPure Quick Gel extraction kit (TransGen Biotech, Beijing, China) PCR product purification
11 pGEM-T vector Ligation of PCR products for sequencing
12 DNA ligation kit (Promega, USA) Ligation of PCR products for sequencing
13 Escherichia coli strain DH5α Transformation for sequencing
14 DEPC-treated water Negative control in real-time PCR