Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV023271 | GenBank Accession | JN632515 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 5253 |
| Definition | Human bocavirus isolate HBov-sh5, complete genome. | Submission Date | 2011-08-28 |
| Strain Name | HBov-sh5 | Sampling Date | 2010 |
| Sampling Country | China | Location | Shanghai Children's Hospital, China |
| Submitted By | Zhao,B., Zhang,X., Shen,J., Teng,Z., Gao,Y. and Yu,X. | Submitting Institution | Microbiology Lab, Shanghai Municipal Center for Disease Control and Prevention, No. 1380 West Zhongshan Road, Changning Disrtict, Shanghai, Shanghai 200336, China |
| Original Host | Homo sapiens | Sample Type | NPAs, stool and blood samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | ReverAid First Strand cDNA Synthesis Kit (Fermentas, CA) | To perform reverse transcription for first strand cDNA synthesis from isolated nucleic acids. | 23638038 | View Related Publication |
| 2 | MagNA Pure LC DNA Isolation Kit (Roche, Germany) | For total nucleic acid extraction from samples using MagNA Pure LC 2.0. | ||
| 3 | Seeplex RV12 ACE detection Kit (Seegene, Germany) | To perform multiplex PCR for detection of 12 common respiratory viruses. | ||
| 4 | HBoV real time PCR kit (Shanghai ZJ Bio-Tech Co., Ltd., People’s Republic of China) | For real time PCR to determine HBoV viral load. | ||
| 5 | Premix Ex Taq Version 2.0 (Takara, Japan) | Used for PCR amplification in synthesizing overlapping fragments of HBoV1 genome. | ||
| 6 | DEPC-treated water | To be used as a solvent in the PCR reactions. | ||
| 7 | Molecular grade water | To be used when preparing PCR reaction mixtures. |