Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV023178 GenBank Accession KT389464
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 1059
Definition Human parvovirus B19 strain HZ117 NS1 gene, partial cds. Submission Date 2015-08-14
Strain Name HZ117 Sampling Date from 2014-01 to 2015-03
Sampling Country China Location Hangzhou, China
Submitted By Zhang,L., Cai,C. and Chen,Z. Submitting Institution The Affiliated Hospital, Hangzhou Normal University, 126 Wenzhou Rd, Hangzhou, Zhejiang 310015, China
Original Host Homo sapiens Sample Type plasma samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Human PV B19, IgM, and IgG ELISA kits (Cat. No.: JM-E15138 and JM-E15139, USA TSZ biological Trade Co., Ltd, North Brunswick, NJ) Detection of B19V IgG and IgM antibodies using enzyme-linked immunosorbent assay (ELISA). 26705119 View Related Publication
2 Passive particle agglutination test kits SERODIA-TPPA and SERODIA-MYCO-II, Fujirebio Inc., Tokyo, Japan Determination of antibodies to M. pneumoniae and T. pallidum using the passive particle agglutination test.
3 TaqMan probe: 50-FAM-CAG ATG CCC TCC ACC CAG ACC TCC AAA C-TAMRA-30 (nt 2,090–2,114) Used in the TaqMan quantitative PCR (qPCR) detection of B19V DNA.
4 B19V-specific primers, synthesized at GenePharma (Shanghai, China) Primers used for the detection of B19V DNA by qPCR.
5 QIAamp DNA Blood Mini Kit Extraction of DNA from plasma for qPCR.
6 Standard controls provided within the ELISA kits Antibody quantification using standard controls.
7 Taq DNA polymerase from Tiangen Biotech Co. Ltd. (Shanghai, China) Amplification of B19V DNA sequences.
8 B19V plasmid pN8 [Zhi et al., 2004], which contains nt 187-5,410 of the J35 isolate Used as a standard by adjusting its concentration with ddH2O in B19V DNA detection.