1
Viral transport media (VTM) consisting of Earle’s Balanced Salt Solution
Used for collecting and transporting stool specimens.
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2
4·4% bicarbonate
Component of the viral transport media (VTM).
3
5% bovine serum albumin (BSA)
Component of the viral transport media (VTM).
4
100 μg/ml vancomycin
Component of the viral transport media (VTM) to prevent bacterial contamination.
5
30 μg/ ml amikacin
Component of the viral transport media (VTM) to prevent bacterial contamination.
6
40 U/ml nystatin
Component of the viral transport media (VTM) to prevent fungal contamination.
7
QIAamp MinElute Virus Spin kit (Qiagen, Germany)
Used for the extraction of total DNA and RNA from stool samples.
8
SuperScript III Reverse Transcriptase (Invitrogen, Life Technology, USA)
Used for the reverse transcription of virus RNA.
9
Random Hexamer primers (Invitrogen, Life Technology, USA)
Used for the reverse transcription of virus RNA.
10
IQ Supermix reaction mixture (Bio-Rad, USA)
Component of the reaction mixture for real-time PCR assays.
11
TaqMan real-time PCR primers: NP1-F and NP1-R
Designed to bind the highly conserved NP1 region of various HBoV strains for screening of HBoV.
12
TaqMan probe: 5′-FAM-AGGAACACCCAATCARCCACCTATCGT-TAMRA-3′
Used as part of the TaqMan real-time PCR for detecting HBoV.
13
Platinum pfx Taq polymerase (Invitrogen, USA)
Used for PCR amplification of HBoV complete genome sequences.
14
Agarose gel DNA purification kit (Bio Teke, China)
Used for purification of PCR products for genome analysis.
15
T-A cloning kit (Takara, China)
Used for cloning PCR products of terminal sequences into vectors for sequencing.
