Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV022884 GenBank Accession HQ267358
Subfamily Parvovirinae Genus Tetraparvovirus
Species Tetraparvovirus primate1 Sequence Length 158
Definition Human parvovirus 4 isolate PRC/4.09/1 ORF1 gene, partial cds. Submission Date 2010-09-18
Strain Name PRC/4.09/1 Sampling Date 2009-04
Sampling Country China Location Shanghai, China
Submitted By Yu,X., Zhang,J., Hong,L., Wang,J. and Zhang,X. Submitting Institution Microbiological Laboratory, Shanghai Municipal Center for Disease Control and Prevention, 1380 West Zhongshan Road, Shanghai 200336, China
Original Host Homo sapiens Sample Type serum sample
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Diagnostic Kit for Anti-HCV IgM, HBsAg and HBeAg Used to assay Anti-HCV IgM, HBsAg, and HBeAg in all serum samples using commercial ELISA kits. 22235298 View Related Publication
2 MagNA Pure LC DNA Isolation Kit Used to isolate total DNA from each serum sample on the MagNA Pure LC 2.0 system.
3 GoTaq PCR Core System II Used to perform PARV4 nested PCR with specific primers.
4 Human Parvovirus (B19) Real Time PCR kit Used to perform real-time PCR for B19 detection in all serum samples.
5 HBoV Real Time PCR kit Used to perform real-time PCR for HBoV detection in all serum samples.
6 Real Time PCR Quantitation kit Used to test serum specimens of chronic HBV patients for the presence of viral DNA.
7 ALT Assay commercial kits Used to assay alanine aminotransferase (ALT) in serum samples from chronic HBV patients.
8 Sequencing services by Biosune Company Used to confirm the identity of nested PCR products by sequencing.