Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV022819 | GenBank Accession | EU370690 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 820 |
| Definition | Human bocavirus isolate HB75 VP1 and VP2 genes, partial cds. | Submission Date | 2008-01-03 |
| Strain Name | HB75 | Sampling Date | from 2006-10 to 2007-07 |
| Sampling Country | China | Location | Guangxi, Shanxi, Jilin, Shanghai, Sichuan, Guangdong, Henan, Hebei, Anhui, Beijing, China |
| Submitted By | Yu,J. | Submitting Institution | Department of Viral Diarreah, Institute for Viral Disease Control and Prevention, 100 Ying-Xin St., Beijing, Beijing 100052, China |
| Original Host | None | Sample Type | fecal samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Phosphate-buffered saline (pH 7.2) | Diluting stool specimens | 18499516 | View Related Publication |
| 2 | QIAamp viral RNA/DNA mini kit (Qiagen) | Extracting total nucleic acid from stool suspensions | ||
| 3 | ELISA kit (IDEIA Rotavirus, Dako) | Identifying Group A rotavirus | ||
| 4 | RT-PCR or PCR | Detecting human astrovirus, norovirus, and adenovirus | ||
| 5 | Forward primer 5′-GGACCACAGTCATCAGAC-3′ (VP1/2-1) | Amplifying an 820-bp VP1/VP2 partial gene fragment of HBoV | ||
| 6 | Reverse primer 5′-CCACTACCATCGGGCTG-3′ (VP1/2-2) | Amplifying an 820-bp VP1/VP2 partial gene fragment of HBoV | ||
| 7 | ExTaq DNA polymerase (Takara Bio) | Performing PCR amplification of the 820-bp VP1/VP2 partial gene fragment | ||
| 8 | 1.8% agarose gel | Visualizing PCR products | ||
| 9 | QIAquick PCR purification kit (Qiagen) | Purifying positive PCR products | ||
| 10 | pGEM-T Easy vector (Promega) | Cloning purified PCR products for sequencing |