Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV022503 | GenBank Accession | KF912802 |
| Subfamily | Parvovirinae | Genus | Erythroparvovirus |
| Species | Erythroparvovirus primate1 | Sequence Length | 994 |
| Definition | Human parvovirus B19 strain B19V-43312/Minsk reg.BLR/08.12 NS1 protein (NS1) and VP1 protein (VP1) genes, partial cds. | Submission Date | 2013-11-28 |
| Strain Name | B19V-43312/Minsk reg.BLR/08.12 | Sampling Date | 2012-02 |
| Sampling Country | Belarus | Location | Belarus |
| Submitted By | Yermalovich,M.A., Semeiko,G.V., Samoilovich,E.O., Huebschen,J.M. and Muller,C.P. | Submitting Institution | Laboratory of vaccine preventable diseases, Research and Practical Center for Epidemiology and Microbiology, Filimonova, 23, Minsk, Minsk 220114, Belarus |
| Original Host | Homo sapiens | Sample Type | serum samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Serum samples | Collected from measles and rubella suspected cases for further testing for Human parvovirus B19 (B19V). | 33441645 | View Related Publication |
| 2 | IgM antibodies against B19V commercial ELISA kits (Biotrin, DRG, Virion\Serion, Euroimmun) | To test the serum samples for IgM antibodies against B19V. | ||
| 3 | QIAamp DNA Blood Mini Kit (Qiagen) | To extract DNA from serum samples. | ||
| 4 | Primers e1855f, e1863f, B19-R1, B19-R2 | For nested-PCR to amplify a 1,100-bp region spanning the NS1/VP1-unique region junction (NS1/VP1u) for genotyping B19V. | ||
| 5 | QIAquick PCR Purification Kit (Qiagen) | For purifying PCR products for sequencing. | ||
| 6 | BigDye Terminator v3.1 Cycle Sequencing Kit (Life Technologies) | For sequencing the purified PCR products. |