Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV022500 | GenBank Accession | MN817225 |
| Subfamily | Parvovirinae | Genus | Erythroparvovirus |
| Species | Erythroparvovirus primate1 | Sequence Length | 994 |
| Definition | Erythroparvovirus primate1 isolate 47879 non-structural protein NS1 (NS) and capsid protein 1 (VP1) genes, partial cds. | Submission Date | 2019-12-10 |
| Strain Name | 47879 | Sampling Date | 2018 |
| Sampling Country | Belarus | Location | Belarus |
| Submitted By | Yermalovich,M.A., Semeiko,G.V., Samoilovich,E.O. and Huebschen,J.M. | Submitting Institution | Laboratory of Vaccine Preventable Diseases, Research and Practical Center for Epidemiology and Microbiology, Filimonova, 23, Minsk 220114, Belarus |
| Original Host | Homo sapiens | Sample Type | serum samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Serum samples | Collected from measles and rubella suspected cases for further testing for Human parvovirus B19 (B19V). | 33441645 | View Related Publication |
| 2 | IgM antibodies against B19V commercial ELISA kits (Biotrin, DRG, Virion\Serion, Euroimmun) | To test the serum samples for IgM antibodies against B19V. | ||
| 3 | QIAamp DNA Blood Mini Kit (Qiagen) | To extract DNA from serum samples. | ||
| 4 | Primers e1855f, e1863f, B19-R1, B19-R2 | For nested-PCR to amplify a 1,100-bp region spanning the NS1/VP1-unique region junction (NS1/VP1u) for genotyping B19V. | ||
| 5 | QIAquick PCR Purification Kit (Qiagen) | For purifying PCR products for sequencing. | ||
| 6 | BigDye Terminator v3.1 Cycle Sequencing Kit (Life Technologies) | For sequencing the purified PCR products. |