Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV022233 | GenBank Accession | MH003649 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 531 |
| Definition | Human bocavirus strain Brazil/2012/AC-12-127 VP1 gene, partial cds. | Submission Date | 2018-02-22 |
| Strain Name | Brazil/2012/AC-12-127 | Sampling Date | from 2011-11 to 2012-11 |
| Sampling Country | Brazil | Location | several hospitals located throughout North Region, Brazil |
| Submitted By | Soares,L.S., Lima,A.B.F., Pantoja,K.C., Lobo,P.S., Cruz,J.F., Guerra,S.F.S., Bezerra,D.A.M., Bandeira,R.S. and Mascarenhas,J.D.P. | Submitting Institution | Virology Section, Evandro Chagas Institute, Br 316, Km 07, S/N, Ananindeua, Para 67020000, Brazil |
| Original Host | Homo sapiens | Sample Type | faecal samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Ridascreen kit (R-Biopharm, Darmstadt, Germany) | Used for the enzyme-linked immunosorbent (ELISA) assay to screen for the presence of rotavirus A (RVA) in faecal samples. | 31215861 | View Related Publication |
| 2 | Buffered Tris-Ca+ 0.01 M | Used to prepare 10% (v/v) faecal suspensions for DNA extraction. | ||
| 3 | Guanidinium isothiocyanate | Used in the silica method for total DNA extraction from faecal suspensions. | ||
| 4 | AK-VP-F1 and AK-VP-R1 primer set | Used for the first round of nested PCR targeting a partial region of the HBoV VP1 gene. | ||
| 5 | AK-VP-F2 and AK-VP-R2 primer set | Used for the second round of nested PCR targeting a partial region of the HBoV VP1 gene. | ||
| 6 | Big Dye Terminator cycle sequencing kit (v 3.1; Applied Biosystems, Foster City, CA, USA) | Used for sequencing the PCR amplicons from HBoV strains. |